GFAP is a 50kD intra-cytoplasmic filamentous protein of the cytoskeleton in astrocytes.
During the development of the central nervous system, it is a cell-specific marker that distinguishes astrocytes from other glial cells.
GFAP immunoreactivity has been shown in immature oligodendrocytes, epiglottic cartilage, pituicytes, papillary meningiomas, myoepithelial cells of the breast and in non-CNS: Schwann cells, salivary gland neoplasms, enteric glia cells, and metastasizing renal carcinomas.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50,000Immunocytochemistry: 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methods
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ul
Host：rabbit
Source Antibody：bovine
Grade：Serum
Purity：serum
Form：Supplied as a lyophilized powder. Reconstitute with sterile ddH2O
Specificity：Recognizes bovine Glial Fibrillary Acidic Protein. Species Crossreactivity: Feline, human, mouse, rat. Predicted to react with other mammals. Predicted length of 432 residues and a MW of 50kD.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Bo Fe Hu Mo Rt
Immunogen：Recombinant protein corresponding to native bovine Glial Fibrillary Acidic Protein expressed in E. coli.
