Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA).
There are two known forms of GAD; GAD65 (65kD) and GAD67 (67kD) that are encoded by two different genes and appear to have distinct intraneuronal distribution in the brain.
This antibody reacts with GAD67 and is predicted to react with GAD65.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.9mg sodium chloride, 0.2mg Na2HPO4, 50% glycerol.
Specificity:Recognizes human GAD67. Species Crossreactivity: Rat. Predicted length of 594 residues and MW of 67kD.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Rt
Immunogen:Synthetic peptide corresponding to ATSSNAGADPNTTNL, aa10-24, from the N-terminal of human GAD67. Species Sequence Homology: Human, mouse