Coronins belong to the WD40 or WD family of proteins.
Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis.
In mammals there are five major coronin proteins, named coronins 1 to 5 in one nomenclature.
Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family).
The mammalian coronin family members are abundant components of eukaryotic cells and each type has a restricted cell type specific expression pattern.
Coronin 1A is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils.
This antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils.
Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type.
Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors.
Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10,000-1:20,000Immunohistochemistry: 1:500-1:1000Immunocytochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.
Specificity：Recognizes Coronin 1a. Species Crossreactivity: Human, feline, mouse, rat. Predicted to react with other mammalian tissue. Predicted length of 461 residues and a MW of 51kD.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Fe Hu Mo Rt
Immunogen：Recombinant full length protein corresponding to Coronin 1a, expressed in E. coli.
