Mitochondrial membrane ATP synthase produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
These ATPases have 2 structural domains: CF1 which is the catalytic core and CF0 which is the membrane proton channel.
CF0 contains 9 subunits including F6.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human ATPase Subunit F6. Species Crossreactivity: Mouse, rat. Predicted length of 108 residues and MW of 13kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa66-81, EYQQELERELFKLKQM from human ATPase Subunit F6.
