Alkaline phosphatase is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules including nucleotides, proteins and alkaloids.
There are four distinct groups of alkaline phosphatases: intestinal, placental, placental-like and tissue non-specific.
The tissue non-specific form is a membrane bound glycosylated protein that is not expressed in any particular tissue.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (formalin fixed/paraffin embedded tissues): 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Specificity：Recognizes human Alkaline Phosphatase. Species Crossreactivity: Mouse, rat. Predicted length of 524 residues and MW of 57kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa135-149, ERSRCNTTQGNEVTS, from human Alkaline Phosphatase, coupled to carrier protein BSA.
