Ulk2 (Serine/threonine-protein kinase Ulk2), otherwise known as Unc-51-like kinase 2, is a widely expressed mammalian Atg1 homologue and member of the protein kinase superfamily, which shares structural similarity with a serine/threonine kinase found in C.
elegans, known to be involved in axonal elongation.
Studies have shown that murine Ulk2 and closely-related Ulk1, localize to autophagic isolation membranes under starvation conditions, and that the binding of Atg13 (Autophagy-related protein 13) to Ulk1 and Ulk2, stabilizes and activates them, and facilitates the phosphorylation of FIP200, a multifunctional protein involved in cell migration, proliferation, apoptosis, and tumor suppression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2.5-5.0ug/ml, detects a band of ~130kD in HEK-293T cell lysates transfected with mouse ULK2
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Immunogen：KLH conjugated synthetic peptide corresponding to aa370-382 from mouse Ulk2. The corresponding sequence differs by 2aa in rat and 3aa in human. Species sequence homology: human and rat
