TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts.
TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors.
Studies have shown that a correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.25-1.0ug/mlWestern Blot: 0.1-0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with 0.5ml sterile PBS, 0.1% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 0.5ml sterile PBS, 0.1% BSA. Labeled with Biotin.
Specificity：Recognizes human TIMP-1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human TIMP-1.
