NQO1 (NAD(P)H:quinone oxidoreductase 1) is a cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones.
NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast.
The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies.
Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukemia has been associated with diminished NQO1 activity and the NQO1 *2 allele.
This product detects a band of ~30kD in human kidney and lung cell lysates.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.03-0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes an epitope within the C-terminal region of human NQO1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo Po Rt
Immunogen：Synthetic peptide, CSIPTDNQIKARK, corresponding to C-terminal region of human NQO1. Species sequence homology: Canine, mouse, porcine, rat
