p38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation.
The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, for the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM sodium fluoride should be added to the sample buffer.
Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.
Specificity：Recognizes rat p38 Mitogen-activated Protein Kinase (p38 MAPK), when phosphorylated at threonine 180 and tyrosine 182. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182. Species sequence homology: bovine, chicken, canine, monkey, mouse and zebrafish
