MAML1 (Mastermind-like Protein 1) is a widely expressed nuclear protein and member of the mastermind family, which acts as a crucial coactivator of Notch-mediated transcriptional activation, and plays a role in hematopoietic development, by acting as a regulator of Notch-mediated lymphoid cell fate decisions.
MAML1 interacts with the Mediator complex component CDK8 (Cyclin-dependent kinase 8), enhancing the phosphorylation and proteolytic turnover of the Notch intracellular domain (NICD) within the nucleus, and forms a DNA-binding complex with RBPSUH/RBP-J kappa/CBF1, playing a vital role in the transcriptional activation of Notch targets.
Studies have shown that the NT region of MAML1 interacts with the DNA-binding domain of the tumor suppressor protein p53, and that over-expression of MAML1, greatly increases p53-mediated gene transcription and biological function.
Detects a band of ~110kD in human skeletal musde cell lysates.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 0.5% BSA 0.02% sodium azide.
Specificity：Recognizes an epitope within the N-terminal region of human MAML1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide sequence, VLPTCPMAEFALPR, corresponding to the N-Terminal region of MAML1 (NP_055572.1). Species sequence homology: Mouse
