Human Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1.
Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukemia and inflammatory bowel disease.
May be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-1RA antibody as the detection reagent and recombinant human IL-1RA as the standard.
May used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-1RA as the positive control.
Applications:Suitable for use in ELISA, Western Blot, Functional Assays.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/mlWestern Blot: 0.1-0.2ug/mlFunctional Assays: 2.5-3.7ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from whole serum.
Form：Supplied as a lyophilized powder in PBS. No preservative added. Reconstitue with 10ul sterile ddH2O. For long term storage the addition of 0.09% sodium azide is recommended.
Specificity：Recognizes human Interleukin-1Ra.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Interleukin-1Ra.
