Specifically Recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system.
Recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody.
Does not block the binding of endothelins
Applications:Suitable for use in Immunofluorescence, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 10-20ug/mlImmunoprecipitation: 20-40ug/mlImmunohistochemistry(Paraffin): 10-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes rat Endothelin B Receptor. Species Crossreactivity: Mouse, human
Isotype：IgG
Calc Applications Abbrev：IF IHC IP
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to KANDHGYDNFRSSNN aa424-437 of rat Endothelin B Receptor, conjugated to KLH.
