ATG3 (Autophagy-related protein 3), otherwise known as APG3, is a widely expressed E2-like enzyme, and member of the ATG3 family, which plays a key role in autophagosome formation, by catalyzing the conjugation of ATG8-like proteins to phosphatidylethanolamine (PE), in conjunction with ATG7.
ATG3 also plays a role in the regulation of mitochondrial homeostasis and cell death, through conjugation with ATG12 at Lys243.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 2.0-4.0ug/mlImmunoprecipitation: 5-10ul Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human ATG3. Species Crossreactivity: Mouse and rat
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa17-32 from human ATG3 conjugated to KLH.
