NALP3 is a cytoplasmic, pyrin-like protein belonging to the NLRP family with a DPIN domain, seven leucine rich repeats and a NACHT domain.
It is known to interact with apoptosis-associated speck-like protein PYCARD/ASC and is a part of the NALP3 inflammasome complex.
NALP3 is involved in the activation of caspase-1 and caspase-5 in response to a number of triggers including bacterial or viral infection, thus leading to the processing of IL-1B and IL-18.
It may also inhibit TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65 and function in the activation of NF-kappa-B signaling, thus regulating inflammation and apoptosis.
Expression is predominantly in peripheral blood leukocytes, polymorphonuclear cells, osteoblasts and chondrocytes.
NALP3 defects leads to several diseases including familial cold auto inflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).
Applications:Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human NLRP3. Expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Peptide corresponding to C-DLYEKAKRDEPK, from an internal region of human NLRP3 (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1).Species Sequence Homology: bovine, canine, mouse, porcine, rat
