GFAP (Glial Fibrillary Acidic Protein) is a member of the intermediate filament protein family which includes desmin, peripherin and vimentin.
It is a major protein component in multiple sclerosis plaques but is found to be up-regulated in other instances where there has been damage or the occurrence of disease to the nervous system.
GFAP is expressed in cells of astrocytic lineage, peripheral ganglia, and non-myelinating Schwann cells.
In addition, neural stem cells also express GFAP.
GFAP runs on SDS-PAGE gels at ~55kD, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Antibodies to GFAP are useful markers of astrocytic cells and neural stem cells.
In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP.
Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene.
All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human GFAP. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-DGEVIKESKQEHKD, from the C-terminal of human GFAPl. Species sequence homology: canine, rat
