The implication of alpha synuclein in neurodegenerative diseases started after the identification of non A beta component of amyloid (NAC peptide) in Alzheimer's disease (AD) brains.
There is now a general group of synucleinopathies which implicates alpha synuclein in Lewy body syndromes, Parkinson' disease and Alzheimer's disease.
Certain missense mutations in the alpha synuclein gene (A53T, A30P) have been linked to the familial Parkinson's disease (PD).
Alpha Synuclein is the major component of Lewy bodies and Lewy neuritis in sporadic PD, dementia with Lewy Bodies and Lewy Body variant of AD.
Lewy bodies are composed of truncated and phosphorylated intermediate neurofilament proteins, alpha synuclein, ubiquitin and associated enzymes.
The synuclein phosphoproteins (15-20kD) are small highly conserved proteins in vertebrates.
The synuclein family includes alpha and beta synucleins and loosely related gamma synuclein and synoretin.
The expression is abundant in neurons and typically is localized at presynaptic terminals.
Antibodies to alpha synuclein provide a specific method to detect Lewy bodies and associated pathological mechanism of PD and AD.
Applications:Suitable for use in Peptide ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Alpha-synuclein. This antibody is expected to recognize reported isoform NACP140 (NP_000336.1) only. Reported variants represent identical protein: NP_000336.1, NP_001139527.1, NP_001139526.1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KDQLGKNEEGAPQE, from the internal region of human Alpha-synuclein (NP_000336.1). Species Sequence Homology: porcine
