Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family.
It is expressed on multiple tissues including fetal neuronal and endothelial cells.
Plexin D1 expression is also induced on tumor vasculature and tumor cells.
The 1879aa mature human Plexin D1 contains a large (1225aa) extracellular domain with a 500aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs.
By alternative splicing, multiple Plexin-D1 isoforms may exist.
Plexin D1 binds Sema4A to suppress angiogenesis.
It also binds Sema3E directly to mediate repulsive vascular vessal guidance.
In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A.
Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human PLXND1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-LAEPKKSHRQSH, from PLXND1, at the internal region of human Plexin D1 (NP_055918.2). Species Sequence Homology: canine, porcine and bovine
