84-2263-91　［取扱停止］ZO1 (Zonula Occludens 1 Protein, Tight junction protein 1, TJP1, DKFZp686M05161, MGC133289, ZO-1) 50ul　214244

特徴

• Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium.
• They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1).
• Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2).
• ZO-1 and -2 are required for tight junction formation and function (3,4).
• In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7).
• The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms.
• ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms.
• While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.