84-2261-46　［取扱停止］VCL (Vinculin, MV, MVCL, CMD1W, CMH15) 50ul　214111

特徴

• Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1-4).
• Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail (1).
• In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation.
• The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration (2).
• Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-alpha and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction (5,6).
• This change in vinculin allows the binding of a number of other proteins, including talin, alpha-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state (2,4).
• Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (7,8).
• This is supported by a recently demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma (9).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.