The TP53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
Activation of TP53 can lead to either cell cycle arrest and DNA repair or apoptosis (1).
TP53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3).
DNA damage induces phosphorylation of TP53 at Ser15 and Ser20 and leads to a reduced interaction between TP53 and its negative regulator, the oncoprotein MDM2 (4).
MDM2 inhibits TP53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6).
TP53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37.
Phosphorylation impairs the ability of MDM2 to bind TP53, promoting both the accumulation and activation of TP53 in response to DNA damage (4,7).
Chk2 and Chk1 can phosphorylate TP53 at Ser20, enhancing its tetramerization, stability, and activity (8,9).
TP53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11).
Phosphorylation of TP53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of TP53 (10,13,14).
TP53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15).
Phosphorylation of TP53 at Ser46 regulates the ability of TP53 to induce apoptosis (16).
Acetylation of TP53 is mediated by p300 and CBP acetyltransferases.
Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes TP53.
Acetylation appears to play a positive role in the accumulation of TP53 protein in stress response (17).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TP53.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human TP53 .
