TNF-a is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells.
It plays a critical role in inflammatory responses and in apoptosis (1).
TNF-a exists as a membrane-anchored and soluble form, both of which show biological activity.
Response to TNF-a is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2).
Antagonists to TNF-a have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3)
The two receptors for TNF-a, TNF-R1 (55kD) and TNF-R2 (75kD) can mediate distinct cellular responses (4,5).
In most cases cytotoxicity elicited by TNF has been reported to act throught TNF-R1 (6,7).
In contrast, TNF-R2 appears to be important in T cell signaling and responses to infection (7,8).
TNF-R2 binds to distinct members of the TRAF family leading to the activation of NF-kB (9,10).
Soluble forms of both receptors have also been characterized which can bind TNF-a and may play an important role in immune disorders (11,12).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TNFRSF1B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human TNFRSF1B.
