84-2255-73　［取扱停止］TNFAIP3 (Tumor Necrosis Factor alpha-induced Protein 3, A20, MGC104522, MGC138687, MGC138688, OTUD7C, TNFA1P2) 100ul　213835

特徴

• A20, also referred to as TNF-alpha-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-kB (1,2).
• It was first identified as a TNF-alpha inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs (3).
• Constitutive expression of A20 is observed in lymphoid tissues (4), but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-alpha (3,5), IL-1 (3,5), phorbol esters (6), and LPS (7).
• Expression of A20 can confer resistance to apoptosis and NF-kB activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members (8,9), and interaction with the NF-kB inhibiting protein ABIN (10).
• Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions (11,12).
• The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation (12).
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.