84-2255-70　［取扱停止］TNF-R1 (TuMOR NECROSIS FACTOR RECEPTOR I, TNF R1, TNFRSF1A, TNFR1, CD120a, TNF R55, FPF) 100ul　213834

特徴

• TNF-alpha is an important cytokine produced by numerous cell types including neutrophils, activated lymphoctyes, macrophages an NK cells.
• It plays a critical role in inflammatory responses and in apoptosis (1).
• TNF-alpha exists as a membrane-anchored and soluble form, both of which show biological activity.
• Response to TNF-alpha is mediated through two receptors, TNF-R1, which is widely expressed, and TNF-R2, which is expressed mainly in immune and endothelial cells (2).
• Antagonists to TNF-alpha have been validated as therapeutic targets for rheumatoid arthritis and other immune disorders (3)
• The two receptors for TNF-alpha, TNF-R1 (55kD) and TNF-R2 (75kD) can mediate distinct cellular responses (4,5).
• In most cases cytotoxicity elicited by TNF has been reported to act through TNF-R1 (6,7).
• Cytotoxicity is mediated by a "death domain" with the intracellular region of the receptor that binds to the death domain adaptor protein TRADD and triggers the activation of caspases (8).
• Soluble forms of both receptors have also been characterized which can bind TNF-alpha and may play an important role in immune disorders (9,10).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.