TNF-a, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2).
Membrane-bound TNF-a is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2).
Both membrane and soluble forms of TNF-a are biologically active.
TNF-a is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1).
Cellular response to TNF-a is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context.
Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-kB) promotes the survival of cells, while TNF-a-mediated activation of caspase-8 leads to programmed cell death (1,2).
TNF-a plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3).
The role of TNF-a in autoimmunity is underscored by blocking TNF-a action to treat rheumatoid arthritis and Crohn’s disease (1,2,4).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TNF.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human TNF .
