Transforming growth factor-beta (TGF-beta) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4).
TGF-beta elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII).
Type I and type II receptors are serine/threonine kinases that form a heteromeric complex.
In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5).
The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-beta with high affinity but lacks a cytoplasmic signaling domain (6,7).
Expression of the type III receptor can regulate TGF-beta signaling through presentation of the ligand to the signaling complex.
The only known direct TGF-beta signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).
Three isoforms of TGF-beta, designated TGF-beta1, TGF-beta2 and TGF-beta3, are encoded by distinct genes and are expressed in a tissue specific manner (10).
Each isoform is synthesized as a larger precursor protein containing a propeptide region that is removed prior to secretion.
Mature TGF-beta contains two polypeptides linked by disulfide bonds to form a protein of approximately 25kD.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human TGFB1. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human TGFB1.
