84-2254-40　［取扱停止］TGFA (Transforming Growth Factor alpha, TGF-alpha, TFGA, Pro-TGF-alpha, TGF-alpha) 50ul　213756

特徴

• Transforming growth factor alpha (TGF-alpha) is a member of the epidermal growth factor (EGF) family, sharing the same receptor, EGFR, and regulating cell proliferation, survival, and differention (1).
• Members of the family share an EGF-like domain of 45-60 amino acids characterized by the conservation of six regularly spaced cysteins, forming three disulfide bonds that function as their receptor binding domain.
• TGF-alpha was initially discovered in the media of retrovirally transformed fibroblasts, and it name comes from its ability to induce transformation in cultured fibroblasts (2).
• This transforming activity was later shown to require TGF-beta, which potentiates the activity of TGF-alpha through a separate receptor (3).
• Soluble TGF-alpha is released from its membrane-bound precusor, pro-TGF-alpha, following protolytic cleavage, but the membrane bound precursor is still able to bind and activate EGFR (4).
• Binding of soluble or membrane bound TGF-alpha to EGFR leads to receptor dimerization, tyrosine autophosphorylation, and activation of downstream signaling components.
• TGF-alpha and related peptides play an important role in the progression of cancer as well as in neuropathological processes (5,6).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.