The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-a and IFN-g (2,3).
Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4).
Stat1 protein exists as a pair of isoforms, Stat1alpha (91kD) and the splice variant Stat1beta (84kD).
In most cells, both isoforms are activated by IFN-alpha, but only Stat1a is activated by IFN-gamma.
The inappropriate activation of Stat1 occurs in many tumors (5).
In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-a and other cellular stresses (6).
Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes STAT1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human STAT1.
