84-2248-75　［取扱停止］SNAI2 (Snail Homolog 2, SLUG, MGC10182, SLUGH1, WS2D) 200ul　213491

特徴

• Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors.
• Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development.
• The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion.
• Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells.
• The tumor suppressor protein p53 induces Slug expression in gamma-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma.
• Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.