Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division.
The cohesin multiprotein complex is required for sister chromatid cohesion.
This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene.
Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain.
Therefore, the encoded protein is thought to be an important part of functional kinetochores.
In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair.
This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Mutations in this gene result in Cornelia de Lange syndrome.
Alternative splicing results in multiple transcript variants encoding different isoforms.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human SMC1A, phosphorylated S957.
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Phospho specific peptide corresponding to residues surrounding S957 of human SMC1A.
