Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-beta/BMP Signaling.
The family consists of nine members that are highly conserved in the N- and C-terminal regions.
Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-beta), an inhibitor for tumor cell proliferation.
Smad3 is activated by activin/TGF-beta receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF-beta target genes.
Interaction of Smad3 with Akt, can initiate TGF-beta induced apoptosis and cell cycle arrest.
Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes SMAD3. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human SMAD3.
