84-2240-50　［取扱停止］RELA (pNF-kappa-B, 65, MGC131774, NFKB3, p65) 100ul　213067

特徴

• Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2).
• There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50), and NF-kB2 (p100/p52).
• Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively.
• Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription.
• In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins (3-5).
• NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression (6-8).
• NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
• NF-kB assembly with IkB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14).
• This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-kB (12-14).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.