84-2239-28　［取扱停止］RAN (GTP Binding Nuclear Protein RAN, GTPase Ran, LPS, RAN Member RAS Oncogene Family, Ras-like Protein TC4, Ras-related Nuclear Protein, ARA24, Gsp1, TC4) 50ul　213005

特徴

• Ran is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells.
• During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus.
• This polarized localization of Ran ensures its role in nuclear transport (1).
• During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2).
• In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3)
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.