The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome.
There are three evolutionarily conserved subunits of PA28: PA28alpha (PSME1), PA28beta (PSME2), and PA28gamma (PSME3) (1,2).
PA28alpha and PA28beta form a heteroheptameric complex and function by binding to the 20S complex at its opening site(s).
The PA28alpha/beta complex is present throughout the cell and participates in MHC class I antigen presentation by promoting the generation of antigenic peptides from foreign proteins (2).
PA28gamma exists in the form of a homoheptamer and is mainly located in the nucleus.
The PA28gamma complex exerts its function by binding and guiding specific nuclear target proteins to the 20S proteasome for further degradation (3,4).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes PSME3. Species Crossreactivity: human, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Rt
Immunogen：Recombinant protein corresponding to human PSME3.
