84-2235-45　［取扱停止］PSMA6 (Proteasome Alpha 6, IOTA, MGC22756, MGC2333, MGC23846, PROS27, p27K) 200ul　212847

特徴

• The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
• It consists of four stacked rings, each with seven distinct subunits.
• The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits.
• While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4).
• Seven different alpha and ten different beta proteasome genes have been identified in mammals (5).
• PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome.
• PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3).
• The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors.
• By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.