Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol.
PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters.
Each member of the PKC family has a specific expression profile and is believed to play a distinct role.
The protein encoded by this gene is one of the PKC family members.
It is a calcium-independent and phospholipid-dependent protein kinase.
This kinase is important for T-cell activation.
It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes PRKCQ, phosphorylated S676. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Phospho specific peptide corresponding to residues surrounding S676 of human PRKCQ.
