This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases.
The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes.
This protein kinase is calcium-independent and phospholipid-dependent.
It is not activated by phorbolesters or diacylglycerol.
This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway.
This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.
There is a single exon pseudogene mapped on chromosome X.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes PRKCI. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human PRKCI.
