84-2230-59　［取扱停止］PKM2 (Pyruvate Kinase, Tumor Type M2, Pyruvate Kinase, Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme, TCB, PK3, PKM2 Pyruvate Kinase, Muscle, CTHBP, PK2) 100ul　212631

特徴

• Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate.
• In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1).
• The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1).
• Studies found that cancer cells exclusively express PKM2 (1-3).
• PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1).
• When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1).
• These cells also show decreased tumorigenicity in mouse xenografts (1).
• Recent studies show that the oncogenic forms of FGFR1 directly phosphorylate Tyr105 of PKM2 and thereby inhibit the formation of active tetrameric PKM2 (4).
• A PKM2 mutant found in cancer cells, in which Tyr105 is replaced by phenylalanine, leads to reduced cell proliferation in hypoxia and tumor growth in xenografts in nude mice (4).
• These findings suggest that the phosphorylation at Tyr105 is a critical switch for the metabolism in cancer cells that promotes tumor growth (4).
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.