Human progesterone receptor (PR) is expressed as two forms: the full length PR B and the short form PR A.
PR A lacks the first 164 amino acid residues of PR B (1,2).
Both PR A and PR B are ligand activated, but differ in their relative ability to activate target gene transcription (3,4).
The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain.
Three sites (Ser81, Ser102, and Ser162) are unique to full length PR B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms (5).
Phosphorylation of PR B at Ser190 (equivalent to Ser26 of PR A) is catalyzed by CDK2 (6).
Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation at Ser190 may be critical to its biological function.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes PGR. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PGR.
