84-2227-52　［取扱停止］PCBP2 (Heterogeneous Nuclear Ribonucleoprotein E2, Poly(rC)-binding Protein 2, HNRPE2, MGC110998, hnRNP-E2) 50ul　212498

特徴

• The protein encoded by this gene appears to be multifunctional.
• Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins.
• The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding.
• Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure.
• It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA.
• The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
• This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2.
• This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes.
• Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2).
• Multiple transcript variants encoding different isoforms have been found for this gene.
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• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.