PARP1, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1).
This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5).
In human PARP1, the cleavage occurs between Asp214 and Gly215, which separates the PARP1 amino-terminal DNA binding domain (24kD) from the carboxy-terminal catalytic domain (89kD) (2,4).
PARP1 helps cells to maintain their viability; cleavage of PARP1 facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000-1:2000Immunohistochemistry: 1:20–1:50Immunocytochemistry: 1:50–1:100Flow Cytometry: 1:20–1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human PARP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human PARP1.
