84-2226-79　［取扱停止］PAI-1 (SERPINE1, PAI, PAI-1, PAI1, PLANH1, Plasminogen activator inhibitor1, Endothelial plasminogen activator inhibitor, Serpin E1) 100ul　212462

特徴

• PAI-1 is a secreted protein that belongs to the serine proteinase inhibitor (serpin) superfamily.
• It inhibits urokinase and tissue plasminogen activators (uPA and tPA) and thus, reduces the conversion of inactive plasminogen to plasmin (1).
• PAI-1 regulates fibrinolysis and plays an important role in vessel patency and tissue remodeling.
• Secreted PAI-1 interacts with the extracellular matrix (ECM) component vitronectin, thereby modulating cell-ECM interactions (2,3).
• PAI-1 is expressed in a variety of tissues with higher expression in liver, vascular endothelial cells, platelets, macrophages, and adipose tissue (1).
• Increased levels of PAI-1 are associated with deep vein thrombosis (4).
• Defects in PAI-1 cause plasminogen activator inhibitor-1 deficiency (PAI-1D), which is characterized by increased bleeding after injury or surgery (5).
• Research studies have shown that high levels of PAI-1 are associated with obesity, aging, insulin resistance, and type 2 diabetes (6-8).
• PAI-1 is transcriptionally regulated by TGF-beta and mediates TGF-beta-induced inhibition of cell migration and invasion in cancer cells (9).
• Studies have shown PAI-1 to be also involved in fibrosis (10).
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:20-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.