The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations.
Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1).
p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3).
DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4).
MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6).
p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37.
Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7).
Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9).
p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11).
Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14).
p53 is phosphorylated at Ser6 and Ser9 by CK1delta and CK1epsilon both in vitro and in vivo (13,15).
Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16).
Acetylation of p53 is mediated by p300 and CBP acetyltransferases.
Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53.
Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17).
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes p53, phosphorylated S15. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Phospho specific peptide corresponding to residues surrounding S15 of human p53.
