The Trk proto-oncogene encodes a 140kD, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues.
Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia.
Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar.
The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF.
Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes.
Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC).
Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:100Immunocytochemistry: 1:50-1:100Immunoprecipitation: 1:20-1:50Flow Cytometry: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes NTRK1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：FC IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human NTRK1.
