NT-4 is a member of the structurally related neurotrophin family of proteins, which includes beta-NGF, BDNF and NT-3 (1).
NT-4 is expressed in a number of cell types and tissues, including neuronal cells, normal breast epithelial cells, melanocytes, activated T cells, and granulocytes (1-5).
NT-4 is required for the development of peripheral sensory neurons (6,7).
NT-4 may be important for the development of long term memory (8).
Increased NT-4 expression in melanoma cells promotes cell proliferation and migration (5).
NT-4 is secreted from cells as a precursor protein, which is proteolytically cleaved into the mature form (1).
NT-4 signaling is mediated through two distinct receptors, the neurotrophin receptor p75NTR and the Trk tyrosine kinase receptor TrkB.
While all neurotrophins bind to the p75NTR receptor, NT-4 preferentially binds to the TrkB receptor (1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes NTF4. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NTF4.
