84-2224-65　［取扱停止］NR1D1 (Nuclear Receptor Subfamily 1 Group D Member 1, Nuclear Receptor Rev-ErbA alpha, EAR1, THRA1, THRAL, EAR-1, HRev) 50ul　212355

特徴

• Reverse orientation c-erbA gene alpha (Rev-erbalpha, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1).
• Rev-erbalpha is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation.
• Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2).
• In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbalpha regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3,4,5,6,7).
• Phosphorylation of Rev-erbalpha Ser55 and Ser59 by GSK3beta appears to stabilize Rev-erbalpha protein levels and is important for synchronizing and maintaining the circadian clock (8).
• Rev-erbalpha also regulates inflammation by targeting the NF-kB responsive genes IL-6 and COX-2 (9).
• Rev-erbalpha lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.