84-2223-97　［取扱停止］NOS3 (Nitric Oxide Synthase 3, eNOS, ECNOS) 200ul　212326

特徴

• Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system.
• It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2).
• The activity of eNOS is regulated by phosphorylation at multiple sites.
• The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3).
• Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5).
• In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7)
• eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113.
• Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunoprecipitation: 1:20-1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.