Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3).
It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1).
Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1).
The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-kB and JNK pathways (4-6).
The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-kB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6).
Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes NOD1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NOD1.
