The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of eight distinct proteins that exhibit different cellular localization.
Members of this group inhibit metastasis in a variety of tumor cell types.
All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events.
Four classes of NDK biochemical activities have been described, including protein-protein interactions, regulation of GTP-binding protein function, DNA-associated activities, and histidine-dependent protein phosphotransferase activity.
NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis.
Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied.
NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity.
Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKa1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKa1.
This regulates AMPKa1 activity towards ACC1, an important regulator of fatty acid metabolism.
Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes human NME1. Species Crossreactivity: mouse and rat
