84-2223-18　［取扱停止］NGFR (Nerve Growth Factor Receptor, CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR) 100ul　212286

特徴

• The p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence and a noncatalytic cytoplasmic domain (1).
• p75NTR displays paradoxical functions when acting alone or with other receptor proteins.
• Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell.
• Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway.
• In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2).The p75NTR protein undergoes sequential cleavage similar to APP and Notch.
• First, alpha-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling.
• At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions.
• gamma-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4).
• Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Immunoprecipitation: 1:20-150Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.