84-2222-22　NEFL (Neurofilament Light, 68kD Neurofilament Protein, CMT1F, CMT2E, NF L, NFL) 200ul　212242

特徴

• The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules.
• Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits.
• Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central alpha-helical rod domain, and a carboxy-terminal tail.
• A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament.
• While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation.
• PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly.
• Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with alpha-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS).
• Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.